Résumé: The main objective of this study was to optimize the parameters that influence the extraction of a cationic dye acid orange 10 (C16H10N2Na2O7S2) from an aqueous solution, by an emulsified liquid membrane (ELM) consisting of Aliquat336 as the extractant, Span80 as the surfactant and cyclohexane as the diluents. The internal phase used was sulphuric acid. The extraction process parameters were studied using a statistical method of experimental Plackett-Burman design. Effects of parameters on the extraction yield were analyzed statistically and a mathematical model of the yield according to different parameters was developed, Main effects were studied and levels of all parameters correspondent to the best yield were determined. The concentration of span80 and the acidity of the internal, the composition and the proportion of the membrane were the most important factors for the extraction yield of AO10. Under optimized operator conditions deduced from main effects, a validation of the model was carried out; the extraction yields given by the polynomial models according to the coded and uncoded parameters and the extraction yields obtained experimentally, were very close comparatively.