Résumé: This work examines the effect of three doses of zinc oxide nanoparticles (ZnONPs), synthetized with Crataegus monogyna leaves using zinc acetate, on the sperm quality of Wistar rats. Animals were divided into 4 groups; the control group maintained without treatment, while ZNP1, ZNP2, and ZNP3 received respectively 10 mg ZNP/kgbw, 50 mg ZNP/kgbw, and 100 mg ZNP/kgbw by gavage for 15 days. Epididymis sperm was collected for sperm parameters: concentration, live sperm, motility, velocity (VCL, VSL, and VAP), linearity (LIN), amplitude lateral head (ALH), and beat cross frequency (BCF). DNA fragmentation was measured in three samples selected from control, ZNP1, and ZNP2. Testicular and epididymis malondialdehyde (MDA), glutathione (GSH), and glutathione peroxidase (GPx) were evaluated. Compared to the control, ZNP1 has a significant reduction of testicular and epididymis weights, sperm concentration, live sperm, motility, VCL, VSL, VAP, LIN, and BCF, with a significant increase of MDA and a significant decrease of GSH levels. The ZNP2 group demonstrated a significant increase in epididymis weight, a raise in sperm parameters (concentration, motility, VCL VSL, VAP, LIN, ALH, and BCF), and an

Résumé: This study investigates the toxic effects of ethanol (Eth) on the reproductive system of male rats and the possible protective role of Silybum marianum seeds-infused solution (SMI) over six consecutive weeks of administration. Animals were divided into the following groups: control, SMI positive control (200 mg/kg/day), Eth1 (1 g/kg/day), Eth2 (2 g/kg/day), Eth1+SMI, and Eth2+SMI. Plasma testosterone concentration, epididymal spermatozoa biology, and testicular and epididymal MDA, GSH and GPx levels were evaluated. The results indicated a significant decrease in testis and epididymis weight, testosterone level, sperm concentration, sperm vitality and sperm motility (total motility, progressive motility, curvilinear velocity, straight-line velocity, velocity average path, beat cross frequency, and lateral head displacement) in both Eth1 and Eth2 compared to the control groups and the combined-treatment groups (Eth1+SMI and Eth2+SMI). Furthermore, results showed a significant elevation in MDA concentration with a significant decrease of testicular and epididymal GSH concentration and GPx activity in theEth1 and Eth2 groups compared to the combined-treatment groups. The administration of SMI succeeded in improving the parameters cited above in the combined-treatment groups compared to the Eth1 and Eth2 groups, and bring them to the levels seen in the control groups. To conclude, SMI has clearly protected reproductive indices against ethanol-induced reprotoxicity in male rats

Résumé: The aim of the study was to evaluate the toxic effects of Nerium oleander roots (R) and leaves (L) aqueous extract on Wistar rat. Serum chemistry, epididymal sperm parameters and histological examination of liver, kidney and brain were assessed after twelve consecutive day’s oral administration of N. oleander. The extracts of R1, R2, L1 and L2 induced no treatment-related adverse effects with regard to general behaviors, hematological, serum chemistry, epididymal sperm parameters and histological profiles of liver, kidney and brain. However, the higher doses (R3, L3) led to, diarrhea, weakness, anorexia, frequent urination, and nasal hemorrhage, with no mortality. The hematological data revealed a significant increase in the levels of hematocrit and platelets in both L3 and R3 compared to the control. However, these two extracts have not affected the level of RBC, hemoglobin, WBC, and lymphocytes, while the L3 has reduced the concentration of glucose and increased that of urea. However, triglycerides, total cholesterol, alanine aminotransferease, albumin, conjugated bilirubin, potassium and calcium levels of L3 and R3 were not statistically significant compared to the control. Simultaneously bilirubin concentration was increased significantly in L3 and R3. There were no significant differences in epididymal sperm analysis when compared to the control. Adverse histological changes were observed in both liver and kidney as well as in the brain. To conclude, N. oleander extracts have disturbed the levels of certain vital variables in rats at higher dose, in which caution should be taken during the use of this plant.

Résumé: In the present study, an attempt was made to evaluate the potential activity of Silybum marianum seeds infused solution (SMI) against ethanol (Eth) toxicity of Wistar rat liver and kidneys. Thirty six males (control, SMI positive control (200 mg/kg bw/day), the Eth1 (01 g/kg bw/day), the Eth2 (02 g/kg bw/day), the Eth1+ SMI and the Eth2+ SMI) were orally administered for six successive weeks to measure serum biochemical parameters as well as hepatic and renal malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GPx) levels. According to the results, the Eth1+ SMI and the Eth2+ SMI alleviated ethanol-induced increased levels of ALT, AST, ALP, total cholesterol, triglycerides, LDL, urea, creatinine and MDA. Furthermore, the co-administration of SMI has maintained hepatic and renal GSH concentration and GPx activity close to that of the control. The SMI has alleviated ethanolinduced toxicity via the improvement of hepatic and renal markers.

Résumé: In the present study, an attempt was made to evaluate the potential activity of Silybum marianum seeds infused solution (SMI) against ethanol (Eth) toxicity of Wistar rat liver and kidneys. Thirty six males (control, SMI positive control (200 mg/kg bw/day), the Eth1 (01 g/kg bw/day), the Eth2 (02 g/kg bw/day), the Eth1+ SMI and the Eth2+ SMI) were orally administered for six successive weeks to measure serum biochemical parameters as well as hepatic and renal malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GPx) levels. According to the results, the Eth1+ SMI and the Eth2+ SMI alleviated ethanol-induced increased levels of ALT, AST, ALP, total cholesterol, triglycerides, LDL, urea, creatinine and MDA. Furthermore, the co-administration of SMI has maintained hepatic and renal GSH concentration and GPx activity close to that of the control. The SMI has alleviated ethanolinduced toxicity via the improvement of hepatic and renal markers.

Résumé: This study explores the promising mitigating activity of fruits (F) and leaves (L) aqueous extract of Crateagus monogyna Jacq. (Fam. Rosaceae) against hepatotoxicity and nephrotoxicity induced by copper sulphate (Cu). Adult male Wistar rats were divided into the control (C), two positive controls ssupplemented with F (1.5g/kg bw/ day) and L (1.5g/kg bw/day) aqueous extract, Cu group (100 mg/kg bw/day),and two other combined groups having the same dosage (Cu+F, Cu+L). The C. monogyna aqueous extracts and copper sulphate were administrated orally for 30 consecutive days, where liver and kidney glutathione (GSH), malondialdehyde (MDA) and glutathione peroxidase (GPx) were evaluated alongside plasma aspartate aminotransaminase(AST), alanine aminotransaminase(ALT),alkaline phosphatase (ALP), urea and creatinine levels. A significant increase in the activity of AST, ALT and ALP and the creatinine level of the Cu group were observed compared to the control, but Cu+F and Cu+L have significantly decreased AST, ALT,ALP, creatinine and urea levels compared to the Cu group. Cu group has respectively increased hepatic MDA concentration, and decreased GSH level and GP x activity compared to the control. The combined treatments (Cu+F and Cu+L)showed a significant decline in MDA concentration, accompanied with significant raise of GSH and GPx levels compared to the Cu group, as well as both positive controls (F and L) demonstrated a significant augmentations of GSH and GPx levels compared to the control. In kidney, Cu group has respectively increased and decreased MDA concentration and GPx activity, but Cu+F and Cu+L have significantly reduced the MDA concentration and raised both GSH level and GPx activity. To conclude, Cu administration to rats has induced hepatotoxicity and nephrotoxicity, while the combination of this metal with the hawthorn aqueous extract have attenuated such toxicity.

Résumé: Objective: The purpose of this study was to investigate the possible beneficial use of common hawthorn Crataegus monogyna aqueous extract at 1.5g/Kg bw/day against chronic copper sulfate intoxication (100mg/Kg bw) in Wistar rats. Material and Method: Animals were divided into 6 groups; the untreated control (C), 2 positive controls treated respectively with hawthorn fruits (F) and leaves (L), 1 group treated with copper (Cu) and 2 combined treatment groups treated with Cu and hawthorn-fruits (CuF) and Cu and hawthorn-leaves (CuL). After 30 days of oral administration, testis weight and plasma testosterone levels were evaluated, in addition to the epididymal sperm concentration, motility, vitality, velocity (VCL, VSL and VAP), the amplitude of lateral head displacement (ALH), and the beat cross frequency (BCF). Testicular glutathione (GSH), malondialdehyde (MDA), and glutathione peroxidase (GPx) were also evaluated. Result and Discussion: Cu exposure reduced testosterone, sperm concentration, live sperm, VCL, VSL, VAP, ALH, BCF, GSH, and GPx levels compared to control groups. Dead sperm and MDA levels were increased in rats of Cu group compared to the untreated control. When compared to the Cu group, levels of testosterone, sperm concentration, sperm motility, live sperm, VCL, VSL, VAP, ALH, BCF, GSH, and GPx were much higher in the CuF and CuL groups, along with a significantly lower MDA concentration. In conclusion, hawthorn, when co-administered as an aqueous extract with Cu, protected most biological markers against copper toxicit, while positive control (s) boosted sperm concentration and velocity (VCL and VAP).