Publications internationales

2022
Oudjani Wided & Bennadja Salima . (2022), Research On The Technological And Genetic Potential Of Durum Wheat In Algeria (Triticum Durum Desf). . NVEO – NATURAL VOLATILES & ESSENTIAL OILS. : Aliva Global Research and Development, https://www.nveo.org/index.php/journal/article/view/5355

Résumé: The present research was concerned with the evaluation of the genetic potential of 20 durum wheat varieties in Algeria by certain biochemical and technological aspects. The aim of this study is therefore to identify and select wheat varieties which are potentially productive in agriculture and which are also characterised by good adaptation, yield stability and production of cereals of good technological and nutritional quality. The different varieties studied indicate the presence of a great variability due to the agro-climatic conditions of the environment; Nevertheless not all genotypes react in the same way some of them are particularly advantaged or on the contrary disadvantaged by certain cultural conditions. The results obtained show the presence of inter-genotype variability for all parameters of technological quality. The protein content at 11% humidity varies from 18.4% in TP/GD/SW which is however a cross, up to 20.1% in the two local varieties Bidi 17 and Hedba 03, Although the germplasm used is quite diversified, between local varieties, improved and introduced, significant variability in protein levels has been recorded within the genotypes studied. In fact, protein content is a low heritability trait and strongly influenced by environmental conditions and cropping practices.Our data for local varieties showed excellent results that have emerged for protein content with little mitadinage in grains, high grain weight and appreciable yields (Exemple : Gemghoum R'ekham and Hedba 3) The introduced varieties show very appreciable results for yield, specific weight With very low speckle and mitadinage levels and average protein levels.( Example: Montepellier variety and Capetit variety 8). Good yields and high grain weights characterising the Simeto, Vitron and Cirta varieties as well as the Daki variety which is well expressed and shows a good yield . we also know that the best qualities are found in local varieties.

L AILANE, A DJAHOUDI, S BENNADJA . (2022), COMPARATIVE STUDY EVALUATING PHYTOCHEMICAL SCREENING, FUNCTIONAL GROUPS ANALYSIS, AND ANTIMICROBIAL ACTIVITY OF TROPAEOLUM MAJUS L. LEAVES, FLOWERS, AND FRUITS. . Plant Archives : Dr. R.S. Yadav, http://www.plantarchives.org/article/5%20COMPARATIVE-STUDY-EVALUATING-PHYTOCHEMICAL-SCREENING,-FUNCTIONAL-GROUPS-ANALYSIS,-AND-ANTIMICROBIAL-ACTIVITY-OF-TROPAEOLUM-MAJUS-L.-LEAVES,-FLOWERS,-AND-FRUITS.pdf

Résumé: Tropaeolum majus is a herbaceous plant that belongs to the Tropaeolaceae family. It is native to the Andesmountain ranges, where it is considered as an important herb in their traditional medicine. However, this plant hasknown, over time, a wide distribution all over the world, including north Africa. Indeed, Tropaeolum majus hasshown in recent years a noteworthy flowering in northeast Algeria, where reigns a Mediterranean climate. The aim ofthis comparative study was to conduct a preliminary assessment of the phytochemical composition and antimicrobialactivity of Tropaeolum majus that grows under the Mediterranean climatic conditions of Annaba city in northeastAlgeria. Thus, a phytochemical screening of 20 metabolites families was performed on free-air dried and pulverizedleaves, flowers, and fruits of this plant using standard qualitative analysis methods based on colour / precipitationreactions. Afterwards, methanolic leaves and flowers extracts, and fruits fixed oil were prepared and served for thefunctional groups analysis using Fourier Transform Infra Red spectroscopy (4000-400 cm-1), as well as theantimicrobial screening using the disc-diffusion method. Apart from narcotics and free anthracene derivatives; thatare absent in the three organs, the phytochemical screening revealed the presence, in Tropaeolum majus, of the soughtmetabolites, mainly phenolic compounds, coumarins, sterols, triterpens, iridoids, and alkaloids. However, the natureand predominance of these metabolites differ between organs, with a unique presence of saponosides in fruits, andthat of free quinones in flowers. Likewise, FTIR analysis revealed absence of infrared radiation absorption in theregion between 2260-2220 cm-1, which indicated absence of toxic cyanide groups; but showed distinct peak values,with a specific infrared radiation absorption in the region located between "1700-700 cm-1", revealing the presence ofvarious functional groups in the three extracts, including aromatic, nitrogen, and sulfur compounds. Tropaeolummajus extracts were found to exert a good antimicrobial activity against tested Gram(+) and Gram(-) germs, especiallyagainst a troublesome food poisoning germ: Bacilluscereus, and the antibiotic-resistant genera: Pseudomonas,Acinetobacter, Staphylococcus, Enterococcus, and Klebsiella. These findings show that Annaba City Mediterraneanclimate factors improved Tropaeolum majus leaves, flowers, and to a second degree fruits nutritional and medicinalqualities enhancing their phytochemical composition as well as antimicrobial properties. Hence, this study suggeststhat this plant could be a potential source for different nutraceutical products that can be used as food additives ordietary supplements. Keywords : Antimicrobial activity, Tropaeolum majus L., Phytochemical screening, Mediterranean climate, FTIR

L Ailane, S Bennadja . (2022), Impact of Mediterranean climate of northeast Algeria on the phytochemical composition and in vitro antiradical power of Tropaeolum majus L. . International Journal of Environmental Studies 80 (3), 716-732 : Taylor & Francis, https://www.tandfonline.com/doi/abs/10.1080/00207233.2022.2099112?journalCode=genv20

Résumé: This paper reports an evaluation of Annaba city climate impact on phytochemical composition and antiradical power of Tropaeolum majus leaf, flower and fruit extracts. Significant phenolic, flavonoid, tannin and carotenoid levels were registered in leaf extract, whereas flower extract showed higher yield, vitamin C and anthocyanin contents. The 2-Diphenyl-1-picrylhydrazyl scavenging power was significantly higher in leaf extract. Conversely, hydroxyl antiradical power was significantly higher in fruit fixed oil. These results show that the Mediterranean climate of Annaba City improved Tropaeolum majus leaves and the nutritional qualities of flowers and fruits, enhancing their phytochemical composition as well as antiradical properties.

2021
Sonia Heni, Hicham Boughendjioua, Salima Bennadja, Abdelghani Djahoudi. (2021), Essential oil composition of Origanum vulgare and its application in substitution of synthetic chemical additives.. Journal of Phytology 2021, 13: 95-100https://doi.org/10.25081/jp.2021.v13.7164

Résumé: In this work, the gas chromatography-mass spectrometry (GC/MS) analysis of the essential oil of Origanum vulgare. L. obtained by hydrodistylation was warried out and it allowed to identify 98.1% of its constituents. The main components are carvacrol (47.6%), thymol (16.6%), p-cymene (13.5%) andγ-terpinene (11.2%). The chemical components of the essence are distributed over five biochemical classes represented mainly by monoterpene phenols and monoterpene carbides. The extraction gave a yield of oil 2.8%. The minimum inhibitory concentration (MIC) of this oil was determined against food spoilage bacteria and pathogenic bacteria belonging to the genus Staphylococcus tested in vitro. This study aims to enhance the value of oregano essential oil by determining its chemical composition on the one hand, and by its application as a natural antibacterial preservative in substitution of synthetic chemical additives on the other hand. The sample was incorporated into a sensitive food matrix exposed to bacterial contamination: white meat. The bacteriostatic/bactericidal power of this bioactive extract was determined by a significant reduction in the number of bacteria and a clear increase in shelf life. Thus, it is possible to propose the use of this aromatic essence as a source of natural preservative.

2020
25. Karima Ounaissia; Salima Bennadja; Laila Aliane et Djahoudi Abdelghani . (2020), Phytochemical screening and anti-bacterial activity of methanolic extracts of the aerial parts of Atriplex halimus L., from biskra (Algeria). . International Journal of Agricultural and Natural Sciences E- ISSN: 2651-3617 13(1): 26-33, 2020.

Résumé: The objectives assigned to the present study are the phytochemical screening of several secondary metabolites and the evaluation of the antibacterial activity of the methanolic extracts of the aerial parts of Atriplex halimus L. Phytochemical screening revealed the presence of substances with high therapeutic values (flavonoids, tannins, polyphenols, coumarin, etc.). The antibacterial activity of the extracts is carried out by the diffusion method on agar medium vis-à-vis sixteen bacterial strains, chosen according to the traditional use of this species in Algeria: Staphylococcus MRSA, Staphylococcus aureus, Staphylococcus ATCC 00, Streptococcus Sp, Enterococci Sp, Enterococci feacalis ATCC 12, Salmonella, E. coli ESBL +, Klebsiella pn Marseille, KpC+, Kp ETP R / IMP R, Serratia Sp, Serratia environmental, Pseudomonas aerogenosa ATCC 53, Pseudomonas VIM 2, Bacillus. The study shows a remarkable antibacterial activity against Gram+ bacteria compared to Gram- bacteria.

Sonia Heni, Hicham Boughendjioua, Meliani Saida, Salima Bennadja and Abdelghani Djahoudi.. (2020), Use of Origanum vulgare Essential Oil as an Antibacterial Additive in the Preservation of Minced Meat. Journal of Pharmaceutical Research International (JPRI), 32(32): 1-9, 202010.9734/jpri/2020/v32i3230927

Résumé: The essential oil extracted by hydrodistillation from the aerial parts of Origanum vulgare L. harvested in the region of Skikda (North-East-Algeria) gave an excellent oil yield (2.8%). Its analysis by Gas Chromatography-Mass Spectrometry (GC/MS) identified 98.10% of its constituents. The major components are: carvacrol (47.6%), thymol (16.6%), p-cymene (13.5%) and γ-terpinene (11.2%). The aim of this study was to the preservative effect of Origanum vulgare essential oil applied to a very low concentration of 0.025% (minimum inhibitory concentration (MIC)), of a sensitive food of Original Research Article Heni et al.; JPRI, 32(32): 1-9, 2020; Article no.JPRI.63389 2 essential nutritional value, of great consumption and easily perishable "minced meat", stored at different times, namely: T 0 = 0 min, T 1 = 40 min, T 2 = 24 h, and T 3 = 48 h. By studying its microbiological quality by determining the rate of reduction of the total aerobic mesophilic microflora and of Staphylococcus aureus. The addition of Origanum vulgare essential oil to minced meat allowed a highly significant reduction of 0.01

2019
Ounaissia, K., Ailane, L., Laredj, H., Bennadja, S. & Smati, D. (2019), Anatomical Study of Atriplex halimus L. (Guettaf) Growing under the Climatic Conditions of Biskra - Algeria. . International Journal of Innovative Approaches in Agricultural Research, 3(4), 698-70510.29329/ijiaar.2019.217.16

Résumé: Atriplex halimus L. Known as “Guettaf” (Amaranthaceae) is used in traditional medicine in Algeria to treat several diseases among them rheumatism and fever. The aim of this work was to study the anatomy of the Atriplex halimus L., growing under the climatic conditions of Biskra (north-east of Algeria). Atriplex halimus L., stem, leaf and root were harvested during the month of January (2019). Microscopic observation of the different organs revealed that the anatomy of this plant contains many more characters of xerophytism as the presence of thick cuticle, trichomes, perivascular sheath and much more hydrophilic cellulosic tissues (parenchyma and collenchymas) than hydrophobic lignified tissues, We can deduce that Atriplex halimus L., requires a high humidity which explains the great growth of this species under the climatic conditions of the region of Biskra. Keywords: Atriplex halimus L., Anatomy, Region of Biskra

2017
Nora Mahfouf, Salima Bennadja, Hichem Nasri, Abdelghani Djahoudi & Sonia Heni . (2017), Antioxidant and Antibacterial Activities of the Essential Oil of Origanum vulgare L. Against Escherichia coli Strains. Journal of Essential Oil Bearing Plants, 20:5, 1391-1399https://doi.org/10.1080/0972060X.2017.1404497

Résumé: The aim of this study is to investigate the antioxidant activity and the antibacterial efficiency of the essential oil extracted by hydrodistillation from leaves of Origanum vulgare against ten (10) clinical strains of Escherichia coli and a reference strain E. coli ATCC provided by the laboratory of microbiology, faculty of medicine, University of Badji Mokhtar (Annaba, Algeria). The chemical composition of the essential oil was characterized by gas chromatography-Mass spectroscopy (GC-MS). Antioxidant activity was evaluated by radical scavenging ability (DPPH method). The antibacterial activity was detected by using disc-diffusion method and the agar dilution method which used for microbial growth inhibition at various concentrations of oil (calculating the minimum inhibitory concentration). The yield of essential oil of O.vulgare was 1.15 % and the major compounds were thymol (32.58 %), γ-terpinen (18.76 %), durenol (17.92 %) and durene (11.40 %). The essential oil showed an antioxidant activity (IC50 = 1.28±0.07 mg/ml) which was lower than the standard antioxidants (ascorbic acid and BHT). It showed a very effective bactericidal activity towards all tested bacterial strains with maximal inhibition zones in the range of 21.3-32.6 mm and a minimum inhibitory concentration (MIC) ranging from 0.314 to 0.628 mg/ ml.

Samira Ati, Bennadja Salima, Boumaraf Warda. (2017), Phytochemical study of three endemic brooms in Algeria: Genista numidica Spach, Genista ferox Poiret and Genista tricuspidata Desf. Int. J. Biosci. 11(3), 178-183
2016
Marwa Bouras, Naffisa Boutefnouchet Abbaci, Salima Bennadja. (2016), Antibacterial Activity of Essential Oil and Aqueous Extract of Eucalyptus globulus Against Methicillin Resistance Staphylococcus aureus and Methicillin Sensitive Staphylococcus aureus. International Journal of Pharmacognosy and Phytochemical Research http://www.ijppr.com/
Marwa Bouras, Naffisa Boutefnouchet Abbaci, Salima Bennadja. (2016), In vitro antibacterial proprieties of aqueous extract and essential oil of Eucalyptus globulus against multi-resistant Klebsiella pneumoniae isolated from hospitalized patients. Der Pharma Chemicawww.derpharmachemica.com
Chouhaira Bouzaâta, Salima Bennadja, Amira Abdesselam. (2016), Valuation of Peels of Four Industrial Tomato Varieties Grown in Annaba (Eastern Algeria). Int. J. Pharm. Sci. Rev. Res.http://www.globalresearchonline.net
2015
Sonia HENI, Salima BENNADJA, Abdelghani DJAHOUDI.. (2015), Chemical composition and antibacterial activity of the essential oil of Thymus ciliatus growing wild in North Eastern Algeria. . Journal of Applied Pharmaceutical Science http://www. Japs online.com
Heni Sonia, Abdesselam Amira, Kermiche Fella, Bennadja Salima, Djahoudi Abdelghani. (2015), Sensibility of Methicillin Resistant Staphylococcus aureus to Essential Oil of Thymus ciliatus. Int. J. Pharm. Sci. Rev. Res
2014
Kouch Mina, Bennadja Salima, Djahoudi Abdelghani, Aouadi Saoudi. (2014), Antipseudomonal Activity of the Essential Oil of Thymus numidicus Poiret. Int. J. Pharm. Sci. Rev. Res
2013
Salima Bennadja, Gérard de Belair & Yasmina Tlili Ait Kaki. (2013), La subéraie de la Numidie orientale : une source de biodiversité.. Quad. Bot. Amb. Applhttp://ortobotanico.unipa.it/quaderni24.html
Y. Tlili-Ait Kaki, S. Bennadja & A. Chefrour. (2013), Revalorisation d’une essence endémique : le sapin de Numidie (Abies numidica). Fl. Medit
Salima Bennadja, Yasmina Tlili Ait Kaki, Abdelghani Djahoudi, Youcef Hadef and Azzedine Chefrour. (2013), Antibiotic activity of the essential oil of Laurel (Laurus nobilis L) on eight bacterial Strains. Journal of Life Sciences
Ouibrahim Amira, Tlili-Ait-kaki Yasmina, Bennadja Salima, Amrouni Sara, Djahoudi Abdelghani and Djebar Mohamed Reda. (2013), Evaluation of antibacterial activity of Laurus nobilis L., Rosmarinus officinalis L. and Ocimum basilicum L from Northeast of Algeria. African Journal of Microbiologiy Research.
2009
Chefrour A, Draiaia R, Tahar A, Ait Kaki Y, Bennadja S and Battesti MJ. (2009), Physicochemical characteristics and pollen spectrum of some North-East Algerian honeys. AJFAND
2007
Chefrour. A, Battesti MJ, Ait Kaki Y, Bennadja S & Tahar A. (2007), Melissopalynologic and Physicochemical Analysis of Some North-East Algerian Honeys. European Journal of Scientific Research
Bennadja Salima, De Belair Gérard & Chefrour Azzedine . (2007), Essai de régénération artificielle par semis de la subéraie de Numidie orientale. Forêt méditerranéenne
2002
Chefrour. A, Bennadja. S, Abdi. A & Tridi. T . (2002), Composition pollinique de l’air d’Annaba (Nord-Est algérien). Allergie et Immunologie

Publications nationales

2015
Amira OUIBRAHIM, Yasmina TLILI-AIT KAKI, Salima BENNADJA, Roukaya MANSOURI, Sabrina AIT KAKI, Samiha KHBIZI et Mohamed-Reda DJEBAR.. (2015), Activité antioxydante et anti-candidosique de l’huile essentielle de Laurus nobilis L. provenant de la région d’El Kala (Nord–Est Algérien). Algerian Journal of Natural Products

Communications internationales

2023
Yasmine Tiah, Amina Benali, Salima Bennadja, Djekoun Abdelhamid et Abdelghani Djahoudi . (2023), Planococcus okeanokoites, a rhizobacterium isolated from the Ezzemoul salt lake, stimulates durum wheat germination and seedlings growth under saline condition. The First International Seminar of Microbiology: Functional Micro-ecology in the service of Living Systems. Tiaret, Algeria, September 20-21, 2023.

Résumé: In Algeria, the agricultural area is mainly located in regions with an arid or semi-arid climate, conductive to soil’s salinization. Salinity is a limiting factor for germination and plant growth. The rhizosphere of halophytes is a source of salt-tolerant bacteria with potential growth promoting activities (PGP). This study aims to assess the ability of P. okeanokoites-isolated from sebkha Ezzemoul-to stimulate the germination characteristics of durum wheat under salt stress. In this study, we have shown that the strain Planococcus okeanokoites- indigenous to highly saline soil of Ezzemoul (Ain M’lila)- is capable to protect wheat seedlings against salt stress. It would be interesting to know its effect on later development stages.

Yasmine Tiah, Amina Benali, Salima Bennadja, Djekoun Abdelhamid et Abdelghani Djahoudi . (2023), Planococcus okeanokoites, a rhizobacterium isolated from the Ezzemoul salt lake, stimulates durum wheat germination and seedlings growth under saline condition. The First International Seminar of Microbiology: Functional Micro-ecology in the service of Living Systems. Tiaret, Algeria, September 20-21, 2023.

Résumé: In Algeria, the agricultural area is mainly located in regions with an arid or semi-arid climate, conductive to soil’s salinization. Salinity is a limiting factor for germination and plant growth. The rhizosphere of halophytes is a source of salt-tolerant bacteria with potential growth promoting activities (PGP). This study aims to assess the ability of P. okeanokoites-isolated from sebkha Ezzemoul-to stimulate the germination characteristics of durum wheat under salt stress.In this study, we have shown that the strain Planococcus okeanokoites-indigenous to highly saline soil of Ezzemoul (Ain M’lila)- is capable to protect wheat seedlings against salt stress. It would be interesting to know its effect on later development stages.

2018
Salima Bennadja. (2018), Chemical composition and antibacterial activity of the essential oil of Citrus aurantium L.. INTERNATIONAL AGRICULTURAL, BIOLOGICAL & LIFE SCIENCE CONFERENCE. Edirne, Turkey
2017
Salima.Bennadja and Samira Ati. (2017), Quantitative analysis and antioxidant activity of three Algerian endemic Genista species. . 112° Congresso della Società Botanica Italiana onlus. Parma (Italy)
2015
Salima Bennadja, Nora Mahfouf and Amira Ouibrahim. (2015), Efficiency of the essential oil of Rosemary against seven bacterial strains.. 11th International Symposium on the Chemistry of Natural Compounds (SCNC 2015) October 01-04, 2015 in Antalya, Turkey
2014
Salima Bennadja and Gerard de Belair. (2014), Barriers to natural regeneration of cork oak in eastern Algeria . Balkan Agricultural Congress 8-11 September 2014, Edirne, turkey